Research Paper Volume 13, Issue 5 pp 7589—7607

Fecal microbiota transplantation mitigates vaginal atrophy in ovariectomized mice

Fecal microbiota transplantation enhances proliferation of vaginal cells. (A). Representative images of PCNA and ESR1 immunohistochemical staining in vaginas from OVX+FMT, OVX+NST, Sham+FMT and Sham+ NST groups, (magnification 200x). (B). Positive rate of PCNA in vaginal epithelial cells in the OVX+FMT (n=11), OVX+NST (n=11), Sham+FMT (n=10) and Sham+NST (n=9) groups (one-way ANOVA, F3, 37=234.68, PC) Positive rate of ESR1 in vaginal epithelial cells in the OVX+FMT (n=11), OVX+NST (n=11), Sham+FMT (n=10) and Sham+NST (n=9) groups (one-way ANOVA, F3, 37=243.5, PD) Serum E1 and E2 level of the OVX+NST group (n=6) and the OVX+FT (n=6) group mice (t-test, P>0.05). Data are shown as mean ± SEM. *P P

Figure 7. Fecal microbiota transplantation enhances proliferation of vaginal cells. (A). Representative images of PCNA and ESR1 immunohistochemical staining in vaginas from OVX+FMT, OVX+NST, Sham+FMT and Sham+ NST groups, (magnification 200x). (B). Positive rate of PCNA in vaginal epithelial cells in the OVX+FMT (n=11), OVX+NST (n=11), Sham+FMT (n=10) and Sham+NST (n=9) groups (one-way ANOVA, F3, 37=234.68, P<0.01). (C) Positive rate of ESR1 in vaginal epithelial cells in the OVX+FMT (n=11), OVX+NST (n=11), Sham+FMT (n=10) and Sham+NST (n=9) groups (one-way ANOVA, F3, 37=243.5, P<0.01). (D) Serum E1 and E2 level of the OVX+NST group (n=6) and the OVX+FT (n=6) group mice (t-test, P>0.05). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01. PCNA: proliferating cell nuclear antigen; ESR1: estrogen receptor alpha; E1: Estrone; E2: Estradiol.