Figure 3. Calycosin regulated cell proliferation by activating or repressing the RP11-65M17.3-ERα loop. (H–M) The effects of calycosin on the RP11-65M17.3-ERα loop were demonstrated by pretreating cells with RP11-65M17.3 shRNA (RI) or MPP. The protein and mRNA expression levels of BRIP1, ERα and RP11-65M17.3 were determined using Western blotting and qRT-PCR. The protein and transcript levels were normalized to the β-actin levels. Representative data from three independent experiments are shown. *p < 0.05 vs. control (0 μM or 0 h); #p < 0.05 vs. 20 μM calycosin alone; $p < 0.05 vs. 10 nM E2 alone.