Research Paper Volume 13, Issue 8 pp 11026—11042

Calycosin stimulates the proliferation of endothelial cells, but not breast cancer cells, via a feedback loop involving RP11-65M17.3, BRIP1 and ERα

Calycosin regulated cell proliferation by activating or repressing the RP11-65M17.3-ERα loop. (H–M) The effects of calycosin on the RP11-65M17.3-ERα loop were demonstrated by pretreating cells with RP11-65M17.3 shRNA (RI) or MPP. The protein and mRNA expression levels of BRIP1, ERα and RP11-65M17.3 were determined using Western blotting and qRT-PCR. The protein and transcript levels were normalized to the β-actin levels. Representative data from three independent experiments are shown. *p #p $p 2 alone.

Figure 3. Calycosin regulated cell proliferation by activating or repressing the RP11-65M17.3-ERα loop. (HM) The effects of calycosin on the RP11-65M17.3-ERα loop were demonstrated by pretreating cells with RP11-65M17.3 shRNA (RI) or MPP. The protein and mRNA expression levels of BRIP1, ERα and RP11-65M17.3 were determined using Western blotting and qRT-PCR. The protein and transcript levels were normalized to the β-actin levels. Representative data from three independent experiments are shown. *p < 0.05 vs. control (0 μM or 0 h); #p < 0.05 vs. 20 μM calycosin alone; $p < 0.05 vs. 10 nM E2 alone.