Research Paper Volume 13, Issue 8 pp 11026—11042

Calycosin stimulates the proliferation of endothelial cells, but not breast cancer cells, via a feedback loop involving RP11-65M17.3, BRIP1 and ERα

Regulation of RP11-65M17.3-ERα loop signaling by calycosin in ECs and BCCs. HUVECs, HMEC-1 cells, MCF-7 cells and T47D cells were pretreated with RP11-65M17.3 shRNA or MPP before incubation with 20 μM calycosin or 10 nM E2 alone. (A–C) The phosphorylation of Akt and ERK1/2 was detected by Western blotting. The corresponding total proteins were used as the internal controls in the same sample. (D–F) The protein and mRNA expression levels of PARP-1 were determined using Western blotting and qRT-PCR. The expression levels were normalized to those of β-actin. Representative data from three independent experiments are shown. *p #p $p 2 alone.

Figure 4. Regulation of RP11-65M17.3-ERα loop signaling by calycosin in ECs and BCCs. HUVECs, HMEC-1 cells, MCF-7 cells and T47D cells were pretreated with RP11-65M17.3 shRNA or MPP before incubation with 20 μM calycosin or 10 nM E2 alone. (AC) The phosphorylation of Akt and ERK1/2 was detected by Western blotting. The corresponding total proteins were used as the internal controls in the same sample. (DF) The protein and mRNA expression levels of PARP-1 were determined using Western blotting and qRT-PCR. The expression levels were normalized to those of β-actin. Representative data from three independent experiments are shown. *p < 0.05 vs. control (0 μM); #p < 0.05 vs. 20 μM calycosin alone; $p < 0.05 vs. 10 nM E2 alone.