Research Paper Volume 13, Issue 6 pp 8320—8334

Celecoxib alleviates zinc deficiency-promoted colon tumorigenesis through suppressing inflammation

Effects of ZD on the expressions of Cyclin D1, PCNA, COX-2 and CXCR2. ZD increased the immunoreactivities of Cyclin D1, PCNA, and COX-2 in the colon of Apcmin/+ mice. (A) Immunoreactivities of Cyclin D1, PCNA, and COX-2 were detected by immunohistochemistry. (B) The data of relative optical density of immunoreactivities. n=6. (C) ZD increased expressions of CXCR2 and COX-2 in the colon of Apcmin/+ mice. Expressions of CXCR2 and COX-2 were detected by Western blot. GADPH was used to normalize the expression level. (D) The data of relative protein expression of Western blot. N=3. Data are showed as mean ± S.D., *P P P

Figure 5. Effects of ZD on the expressions of Cyclin D1, PCNA, COX-2 and CXCR2. ZD increased the immunoreactivities of Cyclin D1, PCNA, and COX-2 in the colon of Apcmin/+ mice. (A) Immunoreactivities of Cyclin D1, PCNA, and COX-2 were detected by immunohistochemistry. (B) The data of relative optical density of immunoreactivities. n=6. (C) ZD increased expressions of CXCR2 and COX-2 in the colon of Apcmin/+ mice. Expressions of CXCR2 and COX-2 were detected by Western blot. GADPH was used to normalize the expression level. (D) The data of relative protein expression of Western blot. N=3. Data are showed as mean ± S.D., *P < 0.05, **P < 0.01 and ***P < 0.001 vs ZS groups.