Research Paper Volume 13, Issue 6 pp 8335—8354

HSCs transdifferentiate primarily to pneumonocytes in radiation-induced lung damage repair

HSC transplantation repaired the radiation-induced lung damage. (A) Transplantation scheme of HSC (CD48-CD150+Lin-Sca-1+c-Kit+) from normal C57BL/6 mice and Atg7-/- mice (Atg7f/f;Vav-iCre mice) into 60Co γ ray 9 Gy lethally irradiated CD45.1 mice. (B) The survival time of mice in each group, including Ctrl (Control non-irradiated mice), IR (Mice irradiated with lethal dose of 9 Gy), HSCT (Transplantation of HSC from normal mice), A7-VAV-T (Transplantation of HSC from Atg7f/f;Vav-iCre mice). N≥10. (C) The lung appearance and coefficient in each group. (D) Lung HE pathological alteration in mice, HSCT alleviated radiation-induced pulmonary inflammatory response. (E) The inflammatory factors including TNF-α, IL-6, IL-1β, IL-10 expression in each group. (F) ALP indicating lung vascular permeability activity in each group. (G) Lung epithelial cells (represented by staining of SP-C and T1-α) and endothelial cells (represented by staining of CD31) percentage in each group. N≥4 in C, D, E, F, G. *: p

Figure 1. HSC transplantation repaired the radiation-induced lung damage. (A) Transplantation scheme of HSC (CD48-CD150+Lin-Sca-1+c-Kit+) from normal C57BL/6 mice and Atg7-/- mice (Atg7f/f;Vav-iCre mice) into 60Co γ ray 9 Gy lethally irradiated CD45.1 mice. (B) The survival time of mice in each group, including Ctrl (Control non-irradiated mice), IR (Mice irradiated with lethal dose of 9 Gy), HSCT (Transplantation of HSC from normal mice), A7-VAV-T (Transplantation of HSC from Atg7f/f;Vav-iCre mice). N≥10. (C) The lung appearance and coefficient in each group. (D) Lung HE pathological alteration in mice, HSCT alleviated radiation-induced pulmonary inflammatory response. (E) The inflammatory factors including TNF-α, IL-6, IL-1β, IL-10 expression in each group. (F) ALP indicating lung vascular permeability activity in each group. (G) Lung epithelial cells (represented by staining of SP-C and T1-α) and endothelial cells (represented by staining of CD31) percentage in each group. N≥4 in C, D, E, F, G. *: p<0.05; **:p<0.01; ***: p<0.001; ****: p<0.0001.