Research Paper Volume 13, Issue 6 pp 8355—8368

90K predicts the prognosis of glioma patients and enhances tumor lysate-pulsed DC vaccine for immunotherapy of GBM in vitro

Combination of apoptosis U251 cells and 90K protein pulsed DC vaccine increased the IFN-γ secretion of CD4+T and CTL cells and the GBM lethal effect of CTL cells. (A) The secretion of IFN-γ in CD4+T cells by different DC vaccines (n=5). (B) The secretion of IFN-γ in CTL cells by different DC vaccines (n=5). (C) The lethal effect of CTL to U251 cells by co-cultured with different DC vaccines by CTL/U251 cells ratio at 25:1, 10:1, 5:1 (n=5). * indicates p value

Figure 7. Combination of apoptosis U251 cells and 90K protein pulsed DC vaccine increased the IFN-γ secretion of CD4+T and CTL cells and the GBM lethal effect of CTL cells. (A) The secretion of IFN-γ in CD4+T cells by different DC vaccines (n=5). (B) The secretion of IFN-γ in CTL cells by different DC vaccines (n=5). (C) The lethal effect of CTL to U251 cells by co-cultured with different DC vaccines by CTL/U251 cells ratio at 25:1, 10:1, 5:1 (n=5). * indicates p value < 0.05; ** indicates p value < 0.01.