Research Paper Volume 13, Issue 5 pp 6346—6358

In vitro P38MAPK inhibition in aged astrocytes decreases reactive astrocytes, inflammation and increases nutritive capacity after oxygen-glucose deprivation

OGD has a higher impact in nutritive loss in aged compared to young astrocytes, whereas aged astrocytes have better anti-oxidant systems. (A) Representative optical microphotograph of young and old primary astrocytes derived from neonatal Wistar rats after 4h of OGD and after 20h of recovery compared to the control group. (B) Representative immunofluorescence and quantification of GFAP positive cells (green) in 1DIV (young) and 30DIV (old) primary astrocyte cell cultures after 4h of OGD and after 20h of recovery compared to the age matched control groups without OGD (n=6). (C) ATP luminescence levels in young and old primary astrocyte cultures after 4h of OGD and after 20h of recovery compared to control groups (n=4). (D–H) Expression of gs, slc1a3, sod2, gclc and igf-1 in young and old astrocytes after 4h of OGD and after 20h of recovery compared to controls (n=6). (I) Protein expression of TNFα in young and old cultured astrocytes after 4h of OGD compared to controls. (J) Representative immunofluorescence and the quantification for CASPASE 3A positive cells in 1DIV (young) and 30DIV (old) astrocytes cell culture derived from neonatal Wistar after 4h of OGD and after 20h of recovery compared to the control group (n=6). Results are expressed as the mean ± SEM. Asterisks denote the significance levels when compared to the control group (***p

Figure 2. OGD has a higher impact in nutritive loss in aged compared to young astrocytes, whereas aged astrocytes have better anti-oxidant systems. (A) Representative optical microphotograph of young and old primary astrocytes derived from neonatal Wistar rats after 4h of OGD and after 20h of recovery compared to the control group. (B) Representative immunofluorescence and quantification of GFAP positive cells (green) in 1DIV (young) and 30DIV (old) primary astrocyte cell cultures after 4h of OGD and after 20h of recovery compared to the age matched control groups without OGD (n=6). (C) ATP luminescence levels in young and old primary astrocyte cultures after 4h of OGD and after 20h of recovery compared to control groups (n=4). (DH) Expression of gs, slc1a3, sod2, gclc and igf-1 in young and old astrocytes after 4h of OGD and after 20h of recovery compared to controls (n=6). (I) Protein expression of TNFα in young and old cultured astrocytes after 4h of OGD compared to controls. (J) Representative immunofluorescence and the quantification for CASPASE 3A positive cells in 1DIV (young) and 30DIV (old) astrocytes cell culture derived from neonatal Wistar after 4h of OGD and after 20h of recovery compared to the control group (n=6). Results are expressed as the mean ± SEM. Asterisks denote the significance levels when compared to the control group (***p<0.001, **p<0.01 and *p<0.05 versus controls, t-test).