Figure 4. Pharmacological inhibition of p38α in aged astrocytes prevents from astrocytes reactivity, inflammatory response and anti-oxidant defense system activation after OGD. (A) Representative optical microphotograph of old primary astrocytes derived from neonatal Wistar after 4h of OGD and treated with PH-797804. (B) MAPK14 gene expression in old astrocyte cultures after 4h of OGD (white), after 4h of OGD with PH-797804 treatment (blue column) in comparison to controls treated with PH-797804 (black) (n=6). (C) Immunoblot of GFAP, TNFα and P-p38MAPK in old primary astrocytes cell culture derived from neonatal Wistar in normoxia and after 4h of OGD and with or without the treatment, PH-797804. (D) Representative quantification of GFAP positive cells in old astrocyte cultures derived after 4h of OGD, after 4h of OGD with PH-797804 treatment, and in control cultures treated with PH-797804 (n=6). (E–K) Expression of gs, slc1a3, sod2, gclc, ngf, igf-1 and il-6 in old astrocyte after 4h of OGD, after 4h of OGD with PH-797804 treatment, in controls treated with PH-797804 (n=6). Results are expressed as the mean ± SEM. Asterisks denote the significance levels when compared to the control group (***p<0.001, **p<0.01 and *p<0.05 versus controls, t-test).