Research Paper Volume 13, Issue 6 pp 8454—8466

Endogenous conversion of n-6 to n-3 polyunsaturated fatty acids facilitates the repair of cardiotoxin-induced skeletal muscle injury in fat-1 mice

High endogenous n-3 PUFA levels attenuates cardiotoxin-induced skeletal muscle injury. (A) Representative images show H&E stained gastrocnemius muscle sections of fat-1 and wild-type mice on days 7 and 14 days after cardiotoxin-induced injury. Scale bar = 100 μm. (B) Quantitative data analysis shows the mean cross-sectional muscle fiber area. (C, D) Representative immunofluorescence images and quantitative data for the intensity of desmin protein expression at 7 days after injury. Scale bar = 50 μm. Data were expressed as the mean ± SD (n = 4-5). NS, non-significant; * P P

Figure 2. High endogenous n-3 PUFA levels attenuates cardiotoxin-induced skeletal muscle injury. (A) Representative images show H&E stained gastrocnemius muscle sections of fat-1 and wild-type mice on days 7 and 14 days after cardiotoxin-induced injury. Scale bar = 100 μm. (B) Quantitative data analysis shows the mean cross-sectional muscle fiber area. (C, D) Representative immunofluorescence images and quantitative data for the intensity of desmin protein expression at 7 days after injury. Scale bar = 50 μm. Data were expressed as the mean ± SD (n = 4-5). NS, non-significant; * P < 0.05, ** P < 0.01 vs. WT.