Figure 5. High endogenous n-3 PUFA levels reduce apoptosis in response to cardiotoxin-induced skeletal muscle injury in fat-1 mice. (A) Representative images show TUNEL staining of the gastrocnemius muscle sections from fat-1 and wild-type mice on days 3 and 7 after CTX-induced injury. Scale bar = 100 μm. (B) Quantitative data for TUNEL-positive cell numbers. (C, D) Representative western blot images and densitometric analysis show the expression levels of Bax, caspase-3, Bcl-2, and Survivin proteins in the gastrocnemius muscles from fat-1 and wild-type mice on day 3 after CTX-induced injury. Data were expressed as the mean ± SD (n = 3-4). NS, non-significant; * P < 0.05, ** P < 0.01 vs. WT.