Figure 7. EPA prevents cardiotoxin-induced C2C12 cell apoptosis. (A) Representative images of morphological change of the differentiated C2C12 cells cultured in the presence and absence of cardiotoxin (0.5 μM) alone and in combination with EPA (50 μM) for 24 h. Scale bar = 100 μm. (B, C) Representative images of TUNEL staining and quantitative data for TUNEL-positive cell numbers. Scale bar = 100 μm. (D, E) Representative western blot images and densitometry analysis shows the levels of Bax, caspase 3, Bcl-2, and Survivin in the lysates of the experimental groups. Data were expressed as the mean ± SD (n = 3). NS, non-significant; ** P < 0.01, *** P < 0.001 vs. Control/CTX+EPA.