Research Paper Volume 13, Issue 5 pp 7644—7659

Platelets transport β-amyloid from the peripheral blood into the brain by destroying the blood-brain barrier to accelerate the process of Alzheimer's disease in mouse models

Effects of tail vein injection of plasma or platelets on AD related indexes of blood and brain tissue in mice. (A) IF staining of brain slices with anti-Iba1 (green) and anti-6E10 (red) antibodies, and the nucleus was stained with DAPI (blue). The arrow indicates the expression of 6E10, which labels the Aβ protein. (B) Fluorescence intensity of 6E10. (C) The number of 6E10 signals. Each group contained 8 mice, and 6 similar coronal sections were selected from each brain for IF staining. Scale bars: high-magnification images = 50 μm, other images = 200 μm. ***PD) Brain image of a C57 mouse by MRI.

Figure 4. Effects of tail vein injection of plasma or platelets on AD related indexes of blood and brain tissue in mice. (A) IF staining of brain slices with anti-Iba1 (green) and anti-6E10 (red) antibodies, and the nucleus was stained with DAPI (blue). The arrow indicates the expression of 6E10, which labels the Aβ protein. (B) Fluorescence intensity of 6E10. (C) The number of 6E10 signals. Each group contained 8 mice, and 6 similar coronal sections were selected from each brain for IF staining. Scale bars: high-magnification images = 50 μm, other images = 200 μm. ***P<0.001 compared with C57+APP/PS1 platelets. (D) Brain image of a C57 mouse by MRI.