Figure 3. VD3 modulates the autophagic flux in IL6-treated C2C12 myotubes. C2C12 myotubes in DM were treated for 24 h with 100 nM VD3, 20ng/mL IL6, or their combination in the presence and absence of 10 μM of chloroquine (CLQ). The autophagosome marker LC3 was detected by western blotting (A) or immunofluorescence (B). Autophagic flux was assessed by quantifying the ratio of LC3 signal in the presence vs. absence of CLQ. For each panel, representative images are on the top and quantification on the bottom. Scale bar, 50 μm. **P < 0.01.