Research Paper Volume 13, Issue 4 pp 4895—4910

Cholecalciferol (vitamin D3) has a direct protective activity against interleukin 6-induced atrophy in C2C12 myotubes

VD3 did not alter the expression of hydroxylases and receptors involved in vitamin D metabolism and activity, and its anti-atrophic action does not depend on intracellular conversion to 25VD. (A) C2C12 myotubes were treated in serum-free medium with VD3 for 24 h, and mRNA levels of Cyp27a1 were assayed by real-time PCR, using liver tissue as a positive control and Gusb as the housekeeping gene. (B) HepG2 cells and C2C12 myotubes were treated in serum-free medium with VD3 for 24 h, and the content of 25VD was quantified in cell lysates with a specific ELISA kit. (C–F) C2C12 myotubes were treated in serum-free medium as above, and mRNA levels of (C) Cyp27b1, (D) Cyp24a1, (E) Vdr, and (F) Pdia3 were assayed by real-time PCR, using Gusb as the housekeeping gene. Data are presented as the mean ± SEM of three independent experiments. ***P

Figure 4. VD3 did not alter the expression of hydroxylases and receptors involved in vitamin D metabolism and activity, and its anti-atrophic action does not depend on intracellular conversion to 25VD. (A) C2C12 myotubes were treated in serum-free medium with VD3 for 24 h, and mRNA levels of Cyp27a1 were assayed by real-time PCR, using liver tissue as a positive control and Gusb as the housekeeping gene. (B) HepG2 cells and C2C12 myotubes were treated in serum-free medium with VD3 for 24 h, and the content of 25VD was quantified in cell lysates with a specific ELISA kit. (CF) C2C12 myotubes were treated in serum-free medium as above, and mRNA levels of (C) Cyp27b1, (D) Cyp24a1, (E) Vdr, and (F) Pdia3 were assayed by real-time PCR, using Gusb as the housekeeping gene. Data are presented as the mean ± SEM of three independent experiments. ***P < 0.001.