Research Paper Volume 13, Issue 6 pp 8588—8598

Inhibition of complement C5a receptor protects lung cells and tissues against lipopolysaccharide-induced injury via blocking pyroptosis

The effect of W-54011 on the expression of proteins related to apoptosis and pyroptosis in lung tissues. (A) The expression of apoptosis-related proteins including Bcl-2, Bax, Caspase 3 and Caspase 11 in different groups was detected using western blotting. (B) The expression of pyroptosis-related proteins including GSDMD, Caspase 1, TLR-4, MyD88 and HMGB1 in different groups was dissected using western blotting. The bar graphs represent the mean gray value of the protein bands after normalized to GAPDH. All data was achieved from 3 times independent replicated experiments. n = 7. *P **P ***P

Figure 4. The effect of W-54011 on the expression of proteins related to apoptosis and pyroptosis in lung tissues. (A) The expression of apoptosis-related proteins including Bcl-2, Bax, Caspase 3 and Caspase 11 in different groups was detected using western blotting. (B) The expression of pyroptosis-related proteins including GSDMD, Caspase 1, TLR-4, MyD88 and HMGB1 in different groups was dissected using western blotting. The bar graphs represent the mean gray value of the protein bands after normalized to GAPDH. All data was achieved from 3 times independent replicated experiments. n = 7. *P < 0.05, **P < 0.01 and ***P < 0.001.