Figure 5. The effect of W-54011 on cell viability and pro-inflammatory cytokines levels in the presence of C5a in Beas-2B cells. (A) The cell viability in different groups was detected by CCK-8 assay. (B) Representative flow cytometry and quantitative analyses for dead cells. (C) The concentrations of pro-inflammatory cytokines including IL-1β, IL-18, IL-6, TNF-α and HMGB1 in supernatant of Beas-2B cells were determined by ELISA assay. Each experiment was repeated at least 3 times. *P < 0.05, **P < 0.01 and ***P < 0.001.