Research Paper Volume 13, Issue 6 pp 8588—8598

Inhibition of complement C5a receptor protects lung cells and tissues against lipopolysaccharide-induced injury via blocking pyroptosis

The effect of W-54011 on the expression of proteins related to apoptosis and pyroptosis in Beas-2B cells. (A) The expression of apoptosis-related proteins including Bcl-2, Bax, Caspase 3, Caspase 4 and Caspase 5 in different groups was detected using western blotting. (B) The expression of pyroptosis-related proteins including GSDMD, Caspase 1, TLR-4, MyD88 and HMGB1 in different groups was analyzed using western blotting. (C) The protein expression of C5aR in Beas-2B cells was analyzed using western blotting. Each experiment was repeated at least 3 times. The bar graphs represent the mean gray value of the protein bands after normalized to GAPDH. All data was achieved from 3 times independent replicated experiments. *P **P ***P

Figure 6. The effect of W-54011 on the expression of proteins related to apoptosis and pyroptosis in Beas-2B cells. (A) The expression of apoptosis-related proteins including Bcl-2, Bax, Caspase 3, Caspase 4 and Caspase 5 in different groups was detected using western blotting. (B) The expression of pyroptosis-related proteins including GSDMD, Caspase 1, TLR-4, MyD88 and HMGB1 in different groups was analyzed using western blotting. (C) The protein expression of C5aR in Beas-2B cells was analyzed using western blotting. Each experiment was repeated at least 3 times. The bar graphs represent the mean gray value of the protein bands after normalized to GAPDH. All data was achieved from 3 times independent replicated experiments. *P < 0.05, **P < 0.01 and ***P < 0.001.