Figure 6. Activation of ATF4 mediates inflammatory cytokines expression in mice TM. (A) mRNA expression of inflammatory cytokines, IL-1α, IL-1β, IL-6 and ELAM-1 in the iridocorneal angle tissues of Ad-ATF4, Ad-GFP and no-injection group (No inj) was determined by real-time RT-PCR 3 days after injection (mean ± SEM, n = 4). ** P<0.01 vs. Ad-GFP, * P<0.05 vs. Ad-GFP. (B) Increased expression of ELAM-1 (red) was detected in the TM of the Ad-ATF4 group 7 days after anterior chamber injection (n = 3). Arrows mark the TM. Blue, nuclear staining with DAPI. BF, bright field. Scale bar, 50 μm.