Research Paper Volume 13, Issue 6 pp 8628—8642

Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAG

Up-regulation of ATF4, ELAM-1 and CHOP in the TM tissues of POAG patients. (A) Frozen sections of human TM tissues of POAG patients (n = 3) or normal controls (n = 4) were double stained with ATF4 (green) and ELAM-1 (red) antibodies. Note the partial colocalization of immunofluorescence in the TM of glaucomatous eyes. (B) Immunohistochemistry showing increased CHOP (green) expression in the TM of POAG patients. Arrows mark the TM. Blue: nuclear staining with DAPI. BF, bright field. Scale bar, 50 μm Supplementary Figure 2.

Figure 7. Up-regulation of ATF4, ELAM-1 and CHOP in the TM tissues of POAG patients. (A) Frozen sections of human TM tissues of POAG patients (n = 3) or normal controls (n = 4) were double stained with ATF4 (green) and ELAM-1 (red) antibodies. Note the partial colocalization of immunofluorescence in the TM of glaucomatous eyes. (B) Immunohistochemistry showing increased CHOP (green) expression in the TM of POAG patients. Arrows mark the TM. Blue: nuclear staining with DAPI. BF, bright field. Scale bar, 50 μm Supplementary Figure 2.