Research Paper Volume 13, Issue 4 pp 4926—4945

Functional analysis of POLD1 p.ser605del variant: the aging phenotype of MDPL syndrome is associated with an impaired DNA repair capacity

Flow cytometry analysis of BrdU-positive cells of WT and MDPL HDFs. Representative histogram of cell cycle profiling, reporting the insets with the relative percentage of cells in different phases of cell cycle (G0/G1, S and G2/M). (I A, I B) indicate 48 hours of cell culture + 6 hours of BrdU. (III A, III B) indicate 72 hours of cell culture + 6 hours of BrdU. (II A, II B) indicate 24hours of cell culture + 24 hours of cisplatin treatment. (IV A, IV B) indicate drug effect after 24 hours from cisplatin removal.

Figure 4. Flow cytometry analysis of BrdU-positive cells of WT and MDPL HDFs. Representative histogram of cell cycle profiling, reporting the insets with the relative percentage of cells in different phases of cell cycle (G0/G1, S and G2/M). (I A, I B) indicate 48 hours of cell culture + 6 hours of BrdU. (III A, III B) indicate 72 hours of cell culture + 6 hours of BrdU. (II A, II B) indicate 24hours of cell culture + 24 hours of cisplatin treatment. (IV A, IV B) indicate drug effect after 24 hours from cisplatin removal.