Figure 4. LCZ696 inhibits LPS-induced expression and production of pro-inflammatory cytokines in HUVECs. Cells were stimulated with LPS in the presence or absence of LCZ696 (10, 20 μM) for 24 hours. (A). mRNA of IL-6; (B). mRNA of IL-1α; (C). mRNA of TNF-β; (D) Secretions of IL-6; (E). Secretions of IL-1α; (F). Secretions of TNF-β (***P < 0.005 vs. control group; ##, ###P < 0.01, 0.005 vs. LPS group).