Research Paper Volume 13, Issue 7 pp 9613—9626

Hypoxia-induced microRNA-155 overexpression in extracellular vesicles promotes renal cell carcinoma progression by targeting FOXO3

miR-155 directly targets FOXO3. (A) Predicted miR-155 binding sites in the FOXO3 3’-UTR were identified using predictive algorithms. (B) The activity of luciferase reporters containing WT or mutant versions of the predicted miR-155 binding site from FOXO3 was measured. (C) The impact of miR-155 on FOXO3 expression was quantified. This experiment was conducted using three distinct biological replicates. *P **P

Figure 6. miR-155 directly targets FOXO3. (A) Predicted miR-155 binding sites in the FOXO3 3’-UTR were identified using predictive algorithms. (B) The activity of luciferase reporters containing WT or mutant versions of the predicted miR-155 binding site from FOXO3 was measured. (C) The impact of miR-155 on FOXO3 expression was quantified. This experiment was conducted using three distinct biological replicates. *P < 0.05, and **P < 0.01.