Figure 6. Senescence-associated secretory phenotype (SASP) factors. (A–C) Interleukin-6 (IL-6; A), tumor necrosis factor-α (TNF-α; B), macrophage migration inhibitory factor (MIF; C) levels were evaluated by ELISA. (D) Representative images of immunofluorescence staining of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB). (E) Nuclear translocation of Nf-κB was evaluated and quantified. Immunofluorescence staining of NF-κB p65 (green) and nuclear with Hoechst 33342 (blue) was performed. (F–G) SMAD2/3 levels were evaluated by western blotting. (H–I) pERK1/2 levels were evaluated by western blotting. (J) Insulin-like growth factor-1 (IGF-1) levels were evaluated by ELISA. (K) Platelet-derived growth factor-BB (PDGF-BB) were evaluated by ELISA. *statistically significant.