Research Paper Volume 13, Issue 8 pp 11061—11082

microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis

miR-363-3p and CD34 expressions are downregulated and NOX4 mRNA expression is upregulated in AS mice and the formation of aortic plaques in AS mice could be prevented by NOX4 knockout. (A) quantification of positive expression of CD34 protein in mice; (B) quantification of the aortic plaque area; (C) the expression of miR-363-3p and NOX4 mRNA as detected by RT-qPCR; The data were analyzed by one-way ANOVA with Tukey's test; n = 10; * p NOX4, NADPH oxidase 4.

Figure 1. miR-363-3p and CD34 expressions are downregulated and NOX4 mRNA expression is upregulated in AS mice and the formation of aortic plaques in AS mice could be prevented by NOX4 knockout. (A) quantification of positive expression of CD34 protein in mice; (B) quantification of the aortic plaque area; (C) the expression of miR-363-3p and NOX4 mRNA as detected by RT-qPCR; The data were analyzed by one-way ANOVA with Tukey's test; n = 10; * p < 0.05 vs. the normal group; # p < 0.05 vs. the AS group; AS, atherosclerosis; NOX4, NADPH oxidase 4.