microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis
Figure 4.miR-363-3p binds to the 3’UTR NOX4 mRNA and inhibits its expression in CAECs and HEK-293 cells. (A) putative miR-363-3p binding sites in the 3’UTR of NOX4 mRNA in the online website (http://www.targetscan.org); (B) miR-363-3p binding with the 3’UTR of NOX4 mRNA in CAECs and HEK-293 cells confirmed by dual luciferase reporter assay. Comparisons between two groups were performed by unpaired t-test, and t-test was adopted for pairwise comparisons. n = 3; * p < 0.05 vs. the mimic NC group; miR, microRNA; NOX4, NADPH oxidase 4; 3’UTR, 3’ untranslated region.