Research Paper Volume 13, Issue 8 pp 11061—11082

microRNA-363-3p reduces endothelial cell inflammatory responses in coronary heart disease via inactivation of the NOX4-dependent p38 MAPK axis

miR-363-3p downregulates protein expression of NOX4 and the ratio of p-p38 MAPK/p38 MAPK in CAECs. (A, B) Representative Western blots of NOX4, p38 MAPK and p-p38 MAPK proteins and their quantitation in CAECs, normalized by β-actin; the data were analyzed by one-way ANOVA with Tukey's post hoc test; n = 3; * p NOX4, NADPH oxidase 4; NC, negative control.

Figure 6. miR-363-3p downregulates protein expression of NOX4 and the ratio of p-p38 MAPK/p38 MAPK in CAECs. (A, B) Representative Western blots of NOX4, p38 MAPK and p-p38 MAPK proteins and their quantitation in CAECs, normalized by β-actin; the data were analyzed by one-way ANOVA with Tukey's post hoc test; n = 3; * p < 0.05 vs. the blank group; # p < 0.05 vs. the miR-363-3p mimic group; & p < 0.05 vs. the miR-363-3p inhibitor group; CAECs, coronary artery endothelial cells; miR, microRNA; NOX4, NADPH oxidase 4; NC, negative control.