Research Paper Volume 13, Issue 7 pp 9732—9747

Comprehensive multiomics analysis of the effect of ginsenoside Rb1 on hyperlipidemia

Comprehensive analysis of microbiology, lipidomics and transcriptomics. (A) Lipidomics showed content of PC increase in normal mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P B) RNA-seq and qRT-PCR showed expression of RNAs associated with glycerophospholipid metabolism. RNA-seq was performed once, PCR were repeated thrice, n=3, data was shown in mean +/- SD. (C) Serum level of hyperlipidemia associated index showed Rb1 reduced total cholesterol level. TG, triglycerides; TC: total cholesterol; LDL, low density lipoprotein; HDL, high density lipoprotein;** represents PD) Lipidomics showed content of PC increase in fecal transplanted germ-free mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P E) QRT-PCR showed that Pmet increased and Dgkg, Phospho1, Pla4g4b decreased in fecal transplanted germ-free mice of Rb1 group. This meant Rb1 increased synthesis and decelerate decomposition of phosphatidylcholine.

Figure 4. Comprehensive analysis of microbiology, lipidomics and transcriptomics. (A) Lipidomics showed content of PC increase in normal mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P <0.05.PC, phosphatidylcholine. POS, positive ion model. NEG, negative ion model. (B) RNA-seq and qRT-PCR showed expression of RNAs associated with glycerophospholipid metabolism. RNA-seq was performed once, PCR were repeated thrice, n=3, data was shown in mean +/- SD. (C) Serum level of hyperlipidemia associated index showed Rb1 reduced total cholesterol level. TG, triglycerides; TC: total cholesterol; LDL, low density lipoprotein; HDL, high density lipoprotein;** represents P<0.01, n=6. (D) Lipidomics showed content of PC increase in fecal transplanted germ-free mice of Rb1 group comparing with that of Model group. Y axis is shown in foldchange, X axis shows different electron cut-off points of PC. All the metabolites with VIP >1 and P <0.05.PC, phosphatidylcholine. (E) QRT-PCR showed that Pmet increased and Dgkg, Phospho1, Pla4g4b decreased in fecal transplanted germ-free mice of Rb1 group. This meant Rb1 increased synthesis and decelerate decomposition of phosphatidylcholine.