Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

TubA attenuates TBHP-induced oxidative stress in chondrocytes. (A–C) Western blotting and quantification of HO-1 and SOD1 in each group. Chondrocytes were treated TBHP or/and TubA for 6 hours. (D, E) Staining with DCFH-DA and quantification in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (F, G) Staining with Mitotracker green and quantification in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P

Figure 3. TubA attenuates TBHP-induced oxidative stress in chondrocytes. (AC) Western blotting and quantification of HO-1 and SOD1 in each group. Chondrocytes were treated TBHP or/and TubA for 6 hours. (D, E) Staining with DCFH-DA and quantification in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (F, G) Staining with Mitotracker green and quantification in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.