Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

Inhibition of HDAC6 by TubA represses ECM degradation in chondrocytes. (A–D) Western blotting and quantification of Collagen II, ADAMTS5 and Aggrecan in each group. Chondrocytes were treated with TBHP or/and TubA for 6 hours. (E, F) IF staining and quantification of MMP13 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (G, H) IF staining and quantification of Collagen II in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P

Figure 4. Inhibition of HDAC6 by TubA represses ECM degradation in chondrocytes. (AD) Western blotting and quantification of Collagen II, ADAMTS5 and Aggrecan in each group. Chondrocytes were treated with TBHP or/and TubA for 6 hours. (E, F) IF staining and quantification of MMP13 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (G, H) IF staining and quantification of Collagen II in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.