Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

Inhibition of HDAC6 by TubA activates autophagy. (A, B) Western blotting and quantification of autophagic markers in each group. Chondrocytes were treated with TubA for 6 hours. (C, D) IF staining and quantification of LC3 in each mouse group, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (E, F) IF staining and quantification of p62 in each mouse group, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (G, H) Chondrocytes were treated with TBHP, TBHP + TubA for 6 hours, the co-localization of LC3 (green) and Lamp2 (red) were captured by IF staining and quantitatively analysed, scale bar = 50 μm, scale bar (enlarged) = 10 μm. N = 5, GAPDH was the loading control, significance: *P

Figure 5. Inhibition of HDAC6 by TubA activates autophagy. (A, B) Western blotting and quantification of autophagic markers in each group. Chondrocytes were treated with TubA for 6 hours. (C, D) IF staining and quantification of LC3 in each mouse group, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (E, F) IF staining and quantification of p62 in each mouse group, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (G, H) Chondrocytes were treated with TBHP, TBHP + TubA for 6 hours, the co-localization of LC3 (green) and Lamp2 (red) were captured by IF staining and quantitatively analysed, scale bar = 50 μm, scale bar (enlarged) = 10 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.