Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

3-MA treatment reverses the effects of TubA on oxidative stress, apoptosis and ECM degradation. (A, B) Western blotting and quantification of LC3, Atg5, HO-1 and SOD1 in each group. Chondrocytes were treated with TBHP + TubA, or TBHP + TubA + 3-MA for 6 hours. (C, D) IF staining and quantification of cleaved caspase3 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (E, F) IF staining and quantification of Collagen II in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (G, H) IF staining and quantification of MMP13 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P

Figure 6. 3-MA treatment reverses the effects of TubA on oxidative stress, apoptosis and ECM degradation. (A, B) Western blotting and quantification of LC3, Atg5, HO-1 and SOD1 in each group. Chondrocytes were treated with TBHP + TubA, or TBHP + TubA + 3-MA for 6 hours. (C, D) IF staining and quantification of cleaved caspase3 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (E, F) IF staining and quantification of Collagen II in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (G, H) IF staining and quantification of MMP13 in each chondrocyte group as above, scale bar = 100 μm, scale bar (enlarged) = 20 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.