Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

TubA inhibits OA development in mice. (A, B) IF staining and quantification of LC3 in each group of OA mice after treatment with TubA, 3-MA, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (C, D) TUNEL staining quantification of positive cells in each group of mice, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (E, F) IF staining and quantification of cleaved caspase3 in each group of mice as above, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (G, H) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. N = 5, GAPDH was the loading control, significance: *P

Figure 7. TubA inhibits OA development in mice. (A, B) IF staining and quantification of LC3 in each group of OA mice after treatment with TubA, 3-MA, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (C, D) TUNEL staining quantification of positive cells in each group of mice, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (E, F) IF staining and quantification of cleaved caspase3 in each group of mice as above, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (G, H) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.