Research Paper Volume 13, Issue 7 pp 9820—9837

Inhibition of HDAC6 by Tubastatin A reduces chondrocyte oxidative stress in chondrocytes and ameliorates mouse osteoarthritis by activating autophagy

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Figure 7. TubA inhibits OA development in mice. (A, B) IF staining and quantification of LC3 in each group of OA mice after treatment with TubA, 3-MA, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (C, D) TUNEL staining quantification of positive cells in each group of mice, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (E, F) IF staining and quantification of cleaved caspase3 in each group of mice as above, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (G, H) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.