**Figure 7.** **TubA inhibits OA development in mice.** (**A**, **B**) IF staining and quantification of LC3 in each group of OA mice after treatment with TubA, 3-MA, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (**C**, **D**) TUNEL staining quantification of positive cells in each group of mice, scale bar = 100 μm, scale bar (enlarged) = 20 μm. (**E**, **F**) IF staining and quantification of cleaved caspase3 in each group of mice as above, scale bar = 50 μm, scale bar (enlarged) = 10 μm. (**G**, **H**) Typical Safranin O staining of the cartilage and subchondral cortical bone in each group of mice, scale bar = 200 μm, scale bar (enlarged) = 50 μm. N = 5, GAPDH was the loading control, significance: *P<0.05, **P<0.01, ***P<0.001.