Research Paper Volume 13, Issue 7 pp 9838—9858

lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis


Figure 6. Dynamic effects of SNHG4 and its target miR-590-3p on CDK1 and CRC cell phenotype. HCT116 and SW620 cells were cotransfected with si-SNHG4 and anti-miR-590-3p, and (A) cell viability was examined by CCK-8 assay; (B) colony formation capacity was examined by colony formation assay; (C) cell cycle progression was examined by flow cytometry; and (D) the protein levels of CDK1, cyclin B1, and cyclin A2 were examined by immunoblotting. *P < 0.05, **P < 0.01, compared to the control group; #P < 0.05, ##P < 0.01, compared to the si-NC (negative control) + anti-miR-590-3p group.