Research Paper Volume 13, Issue 7 pp 9859—9873

Hydrogen sulfide treatment at the late growth stage of Saccharomyces cerevisiae extends chronological lifespan

The late NaHS treatment is more cyto-protective. (A and B) Heat maps of antioxidant DEGs in the early and late NaHS treatments. (C) Venn diagram representing overlapped antioxidant DEGs. (D and E) Heat maps of HSP DEGs in the early and late NaHS treatments. (F) Venn diagram representing overlapped HSP DEGs. (G) The generation of Reactive oxygen species (ROS) in the early and late NaHS treatments at day 5 (120 hours after inoculation) was imaged by fluorescence microscope (left) and quantified by calculating the ratio of positively stained cells (right). (H–J) qPCR analysis of GPX2 (H) HSP78 (I) and HSP104 (J) at 53 hours or 120 hours after inoculation with or without the indicated NaHS treatment. The expression of these genes were normalized with the expression of actin (ACT).*p **p ***p ****p

Figure 5. The late NaHS treatment is more cyto-protective. (A and B) Heat maps of antioxidant DEGs in the early and late NaHS treatments. (C) Venn diagram representing overlapped antioxidant DEGs. (D and E) Heat maps of HSP DEGs in the early and late NaHS treatments. (F) Venn diagram representing overlapped HSP DEGs. (G) The generation of Reactive oxygen species (ROS) in the early and late NaHS treatments at day 5 (120 hours after inoculation) was imaged by fluorescence microscope (left) and quantified by calculating the ratio of positively stained cells (right). (HJ) qPCR analysis of GPX2 (H) HSP78 (I) and HSP104 (J) at 53 hours or 120 hours after inoculation with or without the indicated NaHS treatment. The expression of these genes were normalized with the expression of actin (ACT).*p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001