Figure 5. Metabolic signature obtained through untargeted metabolomic analysis, and illustrated by heatmaps showing the relative abundance changes in metabolites extracted from ileum, colon, liver, and plasma from mice treated with FMT, Akk or with Akk-past (versus PBS or continuous ATB). Untargeted analysis allowed the identification of more than 24000 and 22000 features in the ileum and the colon samples, respectively, while over 3000 and 1700 features were detected in liver and in plasma samples, respectively. Each row represents a detected feature. Hierarchical clustering (euclidean distance, ward linkage method) of the metabolite abundance is shown. PBS, phosphate buffer saline; ATB, antibiotics; FMT, fecal microbiota transplant; Akk, Akkermansia muciniphila; Akk-past, pasteurized Akkermansia muciniphila; FC, fold change.