Figure 2. Overexpression of DNASE1L3 relieves cytoplasmic DNA accumulation under DDR activation. (A) Cytoplasmic accumulation of nuclear DNA in differentially treated cells were assessed, representative images were shown (green, γH2AX; blue, DAPI; Scale bars, 10 μm). (B) Quantification of DNA damage foci (DDF). The number of DDF per cell falls into each of the 0, 1-3, 4-10, and >10 counting categories. At least 100 cells counted per group. (C, D) qPCR analysis of chromosomal DNA in cytoplasmic fraction of cells treated in different group.