Research Paper Volume 13, Issue 7 pp 9874—9899

DNASE1L3 arrests tumor angiogenesis by impairing the senescence-associated secretory phenotype in response to stress

Overexpression of DNASE1L3 relieves cytoplasmic DNA accumulation under DDR activation. (A) Cytoplasmic accumulation of nuclear DNA in differentially treated cells were assessed, representative images were shown (green, γH2AX; blue, DAPI; Scale bars, 10 μm). (B) Quantification of DNA damage foci (DDF). The number of DDF per cell falls into each of the 0, 1-3, 4-10, and >10 counting categories. At least 100 cells counted per group. (C, D) qPCR analysis of chromosomal DNA in cytoplasmic fraction of cells treated in different group.

Figure 2. Overexpression of DNASE1L3 relieves cytoplasmic DNA accumulation under DDR activation. (A) Cytoplasmic accumulation of nuclear DNA in differentially treated cells were assessed, representative images were shown (green, γH2AX; blue, DAPI; Scale bars, 10 μm). (B) Quantification of DNA damage foci (DDF). The number of DDF per cell falls into each of the 0, 1-3, 4-10, and >10 counting categories. At least 100 cells counted per group. (C, D) qPCR analysis of chromosomal DNA in cytoplasmic fraction of cells treated in different group.