Research Paper Volume 13, Issue 7 pp 9874—9899

DNASE1L3 arrests tumor angiogenesis by impairing the senescence-associated secretory phenotype in response to stress

DNASE1L3 impairs angiogenesis through the differential expression of SASP under DDR activation. (A) Tissue microarray was stained with anti-DNASE1L3 and anti-CD34 antibody, low expression of DNASE1L3 was considerably associated with cancer vasculature invasion. (scale bar, 100 μm). (B, C) Tumor tissues of DNASE1L3 intermediately or highly expressed were observed to be linked with lower vasculature invasion. (scale bar, 100 μm). (D) The histological scores of DNASE1L3 and the CD34 Chalkley counts in HCC tissues was analyzed and showed a significant negative association. (E, F) The motility of HUVECs were assessed by wound healing assay, the supernatants from cells in differently treated groups were added into the culture of HUVECs, images were taken at 0h and 24h (scale bar, 100 μm). (G, H) The cellular migration ability of HUVECs were determined by the transwell migration assay. The supernatants from cells in differently treated groups were added into the lower chamber, images were taken after 24h of incubation (scale bar, 100 μm). (I, J) The tube formation ability of HUVECs were determined by tube formation assay. The supernatants from cells in differently treated groups were added into the culture, images were taken after 6h of incubation (scale bar, 100 μm). The results show the means ± SD from at least three separate experiments.

Figure 4. DNASE1L3 impairs angiogenesis through the differential expression of SASP under DDR activation. (A) Tissue microarray was stained with anti-DNASE1L3 and anti-CD34 antibody, low expression of DNASE1L3 was considerably associated with cancer vasculature invasion. (scale bar, 100 μm). (B, C) Tumor tissues of DNASE1L3 intermediately or highly expressed were observed to be linked with lower vasculature invasion. (scale bar, 100 μm). (D) The histological scores of DNASE1L3 and the CD34 Chalkley counts in HCC tissues was analyzed and showed a significant negative association. (E, F) The motility of HUVECs were assessed by wound healing assay, the supernatants from cells in differently treated groups were added into the culture of HUVECs, images were taken at 0h and 24h (scale bar, 100 μm). (G, H) The cellular migration ability of HUVECs were determined by the transwell migration assay. The supernatants from cells in differently treated groups were added into the lower chamber, images were taken after 24h of incubation (scale bar, 100 μm). (I, J) The tube formation ability of HUVECs were determined by tube formation assay. The supernatants from cells in differently treated groups were added into the culture, images were taken after 6h of incubation (scale bar, 100 μm). The results show the means ± SD from at least three separate experiments.