Figure 7. DNASE1L3 arrests tumor angiogenesis by regulating the senescence-associated secretory phenotype in response to stress. (A) Matrigel plugs supplemented with cell supernatants from differently treated groups were subcutaneously injected into mice. (B) Hemoglobin content was assessed at day 10 after injection. (C) Representative images of CD34 staining of the matrigel plugs in differently treated groups (scale bar, 200 μm). Gross view observation of the matrigel plugs were shown in the bottom right corner of each figure. (D) CD34 positive area was evaluated in each group. (E) Constructed subcutaneous tumor model was used to investigate the role of DNASE1L3 in tumor angiogenesis. (F) Vascular density was quantified by CD34 Chalkley count under IHC. (G) Representative images of CD34 staining of subcutaneous tumors in differently treated groups (scale bar, 200 μm).