Research Paper Volume 13, Issue 5 pp 6406—6419

Ablation of CRBN induces loss of type I collagen and SCH in mouse skin by fibroblast senescence via the p38 MAPK pathway

The absence of CRBN in mice skin mimics UV-damaged phenotype. (A) Representative image of Masson’s trichrome stained section showing the skin structure affected by ultraviolet irradiation after 4 weeks. (B) Analysis of the level of collagen fibers in the skin section in (A). (C) Western blots analysis using the protein lysate from the mouse skin. Proteins were subjected to immunoblotting using the anti-CRBN, anti-MMP1, anti-Collagen type1, and β -actin antibodies. The β-actin was used as a loading control. (D) Relative band intensities determined by densitometric-analysis of each protein in blot (C). The results shown are representative images of independent experiments (n=5). Scale bar = 100μm. *P P P

Figure 2. The absence of CRBN in mice skin mimics UV-damaged phenotype. (A) Representative image of Masson’s trichrome stained section showing the skin structure affected by ultraviolet irradiation after 4 weeks. (B) Analysis of the level of collagen fibers in the skin section in (A). (C) Western blots analysis using the protein lysate from the mouse skin. Proteins were subjected to immunoblotting using the anti-CRBN, anti-MMP1, anti-Collagen type1, and β -actin antibodies. The β-actin was used as a loading control. (D) Relative band intensities determined by densitometric-analysis of each protein in blot (C). The results shown are representative images of independent experiments (n=5). Scale bar = 100μm. *P < 0.05; **P < 0.01; ***P < 0.005; n.s., not significant.