Research Paper Volume 13, Issue 7 pp 9900—9910

LncRNA MNX1-AS1 drives aggressive laryngeal squamous cell carcinoma progression and serves as a ceRNA to target FoxM1 by sponging microRNA-370

Knockdown of MNX1-AS1 inhibits LSCC growth in vitro and in vivo. (A) qRT-PCR analysis of MNX1-AS1 expression in human LSCC cell line TU-212 and normal bronchial epithelial cell line (16HBE). (B) qRT-PCR analysis of MNX1-AS1 expression in TU212 cells transfected with sh-NC and sh-MNX1-AS1. (C, D) cell proliferation and colony formation were determined in TU212 cells transfected with sh-NC and sh-MNX1-AS1. (E–F) The volume and weight of tumor from nude mice model were measured. (G) IHC assay was used to evaluate the protein expression of Ki-67 in tumor. *P **P

Figure 2. Knockdown of MNX1-AS1 inhibits LSCC growth in vitro and in vivo. (A) qRT-PCR analysis of MNX1-AS1 expression in human LSCC cell line TU-212 and normal bronchial epithelial cell line (16HBE). (B) qRT-PCR analysis of MNX1-AS1 expression in TU212 cells transfected with sh-NC and sh-MNX1-AS1. (C, D) cell proliferation and colony formation were determined in TU212 cells transfected with sh-NC and sh-MNX1-AS1. (E–F) The volume and weight of tumor from nude mice model were measured. (G) IHC assay was used to evaluate the protein expression of Ki-67 in tumor. *P < 0.05; **P < 0.01.