Figure 4. JAG1 mediates the effect of miR-512-5p on GBM cells. (A) JAG1 expression determined by western blotting after transfecting U87-miR-512-5p or LN229-anti-miR-512-5p cells with JAG1 or sh-JAG1. (B) Cell proliferation activity as examined by EdU assays. (200 ×) (C) Cell proliferation activity as examined by colony formation assays. (D) Analysis of cell cycle by flow cytometry. (E) G1-arrest-relevant cell cycle regulators determined by western blotting. Data are represented as the mean ± standard deviation of three independent experiments. *p < 0.05, **p < 0.01, Student's t-test, compared to the Control group.