Research Paper Volume 13, Issue 7 pp 9991—10014

Transcriptome and lipidome profile of human mesenchymal stem cells with reduced senescence and increased trilineage differentiation ability upon drug treatment

General workflow of the strategy established in this study. Cells such as hMSCs were treated with indicated drugs and the phenotypes were observed and determined which was used to classify the effect of drugs. Then the cells and supernatants which was collected at indicated time points were subjected to the lipidomics and transcriptomics analysis by MS. Furthermore, the altered genes, pathways and lipids were further analyzed based on the results of MS and to narrow down the underlying mechanisms for the drugs in inducing the observed phenotypes.

Figure 7. General workflow of the strategy established in this study. Cells such as hMSCs were treated with indicated drugs and the phenotypes were observed and determined which was used to classify the effect of drugs. Then the cells and supernatants which was collected at indicated time points were subjected to the lipidomics and transcriptomics analysis by MS. Furthermore, the altered genes, pathways and lipids were further analyzed based on the results of MS and to narrow down the underlying mechanisms for the drugs in inducing the observed phenotypes.