Research Paper Volume 13, Issue 6 pp 9071—9084

Long non-coding RNA THRIL inhibits miRNA-24-3p to upregulate neuropilin-1 to aggravate cerebral ischemia-reperfusion injury through regulating the nuclear factor κB p65 signaling

LncRNA THRIL regulates NF-κB p65 signaling pathway through miR-24-3p, and further regulates OGD/R-induced neuronal apoptosis and inflammatory response. (A) SH-SY5Y cells were transfected and divided into six groups: control, OGD/R, OGD/R+pcDNA3.1-NC, OGD/R+pcDNA3.1-THRIL, OGD/R+NC-mimic, and OGD/R+pcDNA3.1-THRIL+miR-24-3p-mimic groups. (A) Cellular activity was identified by CCK-8. (B) The concentrations of inflammatory factors (IL-6, IL-1β, TNFα) were examined by ELISA. (C) The expression levels of inflammatory factors (IL-6, IL-1β, TNFα) were detected by RT-qPCR. (D) TUNEL was performed to evaluate cell apoptosis. (E) The expression levels of apoptotic proteins were detected by western blot. (F) The p65 protein expression was detected by western blot. Data are shown as mean ± SD for three-independent experiments. **P

Figure 6. LncRNA THRIL regulates NF-κB p65 signaling pathway through miR-24-3p, and further regulates OGD/R-induced neuronal apoptosis and inflammatory response. (A) SH-SY5Y cells were transfected and divided into six groups: control, OGD/R, OGD/R+pcDNA3.1-NC, OGD/R+pcDNA3.1-THRIL, OGD/R+NC-mimic, and OGD/R+pcDNA3.1-THRIL+miR-24-3p-mimic groups. (A) Cellular activity was identified by CCK-8. (B) The concentrations of inflammatory factors (IL-6, IL-1β, TNFα) were examined by ELISA. (C) The expression levels of inflammatory factors (IL-6, IL-1β, TNFα) were detected by RT-qPCR. (D) TUNEL was performed to evaluate cell apoptosis. (E) The expression levels of apoptotic proteins were detected by western blot. (F) The p65 protein expression was detected by western blot. Data are shown as mean ± SD for three-independent experiments. **P < 0.01.