Research Paper Volume 13, Issue 8 pp 11135—11149

WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA

4-PBA protects AC16 cells from apoptosis and endoplasmic reticulum stress induced by WTAP overexpression. (A) The m6A levels in AC16 cells transduced with WTAP-overexpressing plasmid and treated with 2 mM 4-PBA for 48 h were measured by ELISA. (B, C) Cell apoptosis and (D, E) expression of WTAP, PARP, cleaved Caspase-3, ATF4 and CHOP. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P ##P ###P

Figure 2. 4-PBA protects AC16 cells from apoptosis and endoplasmic reticulum stress induced by WTAP overexpression. (A) The m6A levels in AC16 cells transduced with WTAP-overexpressing plasmid and treated with 2 mM 4-PBA for 48 h were measured by ELISA. (B, C) Cell apoptosis and (D, E) expression of WTAP, PARP, cleaved Caspase-3, ATF4 and CHOP. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P < 0.001 vs vector. ##P < 0.01, ###P < 0.001 vs WTAP.