Research Paper Volume 13, Issue 8 pp 11135—11149

WTAP promotes myocardial ischemia/reperfusion injury by increasing endoplasmic reticulum stress via regulating m6A modification of ATF4 mRNA

ATF4 regulates the transcription of WTAP. AC16 cells were transduced with ATF4 shRNA and treated with H/R for 48 h. (A) WTAP promoter activity and (B–D) WTAP expression. (E) ATF4 binding site (BS) in WTAP promoter schematic diagram. (F) Chromatin immunoprecipitation assay of ATF4 binding with WTAP. (G) Chromatin fragments from AC16 cells transduced with ATF4 shRNA and H/R were immunoprecipitated to analyze the binding of ATF4 to WTAP. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P ###P

Figure 5. ATF4 regulates the transcription of WTAP. AC16 cells were transduced with ATF4 shRNA and treated with H/R for 48 h. (A) WTAP promoter activity and (B–D) WTAP expression. (E) ATF4 binding site (BS) in WTAP promoter schematic diagram. (F) Chromatin immunoprecipitation assay of ATF4 binding with WTAP. (G) Chromatin fragments from AC16 cells transduced with ATF4 shRNA and H/R were immunoprecipitated to analyze the binding of ATF4 to WTAP. All experiments were repeated at least three times, and data are represented as mean ± SD. ***P < 0.001 vs control (untreated). ###P < 0.001 vs H/R+shNC.