Research Paper Volume 13, Issue 10 pp 13515—13534

Suppressing the KIF20A/NUAK1/Nrf2/GPX4 signaling pathway induces ferroptosis and enhances the sensitivity of colorectal cancer to oxaliplatin

High KIF20A expression in resistant CRC cell line suppressed the intracellular ferroptosis process. (A) Correlation between expression levels of KIF20A and GPX4 in colorectal cancer samples. (B) The expression level of KIF20A of colorectal cancer patients in different stages. (C) The expression level of KIF20A in different colorectal cancer cell lines were examined by WB assay. (D) WB assay was used to observe whether KIF20A silencing could impact the intracellular GPX4 expression level. Top, HCT116-Or cells. Bottom, H716 cells. (E, F) HCT116-Or (E) and H716 (F) cells were selected to construct the subcutaneous xenograft model of nude mice, so as to observe whether KIF20A silencing would affect the suppression of oxaliplatin on colorectal cancer in vivo. Top, representative images of xenografted tumor in the indicated groups. Bottom, statistical results of growth of xenografted tumor with time. The data are presented as the mean ± SD, ***p

Figure 2. High KIF20A expression in resistant CRC cell line suppressed the intracellular ferroptosis process. (A) Correlation between expression levels of KIF20A and GPX4 in colorectal cancer samples. (B) The expression level of KIF20A of colorectal cancer patients in different stages. (C) The expression level of KIF20A in different colorectal cancer cell lines were examined by WB assay. (D) WB assay was used to observe whether KIF20A silencing could impact the intracellular GPX4 expression level. Top, HCT116-Or cells. Bottom, H716 cells. (E, F) HCT116-Or (E) and H716 (F) cells were selected to construct the subcutaneous xenograft model of nude mice, so as to observe whether KIF20A silencing would affect the suppression of oxaliplatin on colorectal cancer in vivo. Top, representative images of xenografted tumor in the indicated groups. Bottom, statistical results of growth of xenografted tumor with time. The data are presented as the mean ± SD, ***p < 0.001 (versus shMOCK+Oxaliplatin).