Figure 4. Effect of miR-27 and miR-195 inhibition on hypoxia-influenced MR. Isolated cardiomyocytes were transfected with NC, miR-27, and miR-195 inhibitors for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR was performed to show the mRNA expression levels of (A) SIRT1, (B) FOXO1, (C) SREBP1c, (D) ACLY, (E) ACACB, (F) ADPN, (G) ATGL, and (H) GLUT4 in the isolated cardiomyocytes in each group (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the normoxia group. #P < 0.05 and ##P < 0.01 vs. the hypoxia group.