Research Paper Volume 13, Issue 7 pp 10158—10174

Hypoxia-induced miR-27 and miR-195 regulate ATP consumption, viability, and metabolism of rat cardiomyocytes by targeting PPARγ and FASN expression

Effect of miR-27 and miR-195 inhibition on the viability of hypoxia-induced cardiomyocytes. Isolated cardiomyocytes were transfected with NC, miR-27, and miR-195 inhibitors for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. (A) EdU staining was performed to detect the proliferation of cells in each group. (B) The CCK-8 assay revealed the cell viability in each group. (C) Annexin V-FITC/PI flow cytometry was utilized to detect the apoptotic proportion of cardiomyocytes. (D) WB was utilized to detect the levels of Bax, caspase-9, and cleaved caspase-9 (n = 3). *P #P ##P

Figure 5. Effect of miR-27 and miR-195 inhibition on the viability of hypoxia-induced cardiomyocytes. Isolated cardiomyocytes were transfected with NC, miR-27, and miR-195 inhibitors for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. (A) EdU staining was performed to detect the proliferation of cells in each group. (B) The CCK-8 assay revealed the cell viability in each group. (C) Annexin V-FITC/PI flow cytometry was utilized to detect the apoptotic proportion of cardiomyocytes. (D) WB was utilized to detect the levels of Bax, caspase-9, and cleaved caspase-9 (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the normoxia group. #P < 0.05 and ##P < 0.01 vs. the hypoxia group.