Research Paper Volume 13, Issue 7 pp 10158—10174

Hypoxia-induced miR-27 and miR-195 regulate ATP consumption, viability, and metabolism of rat cardiomyocytes by targeting PPARγ and FASN expression

miR-27 and miR-195 targeted the 3’-UTRs of PPARγ and FASN. (A) Bioinformatic analysis showed that the 3’-UTRs of PPARγ and FASN had binding sites in the sequences of miR-27 and miR-195. (B, C) The DLRA was performed by co-transfection of luciferase reporter containing the WT or MU-binding sites of PPARγ and FASN with miR-27 and miR-195 mimics into cardiomyocytes. (D–F) Isolated cardiomyocytes were transfected with NC, miR-27, and miR-195 inhibitors for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR and WB were utilized to detect the expression levels of PPARγ and FASN mRNA and protein in isolated cardiomyocytes in each group (n = 3). **P ###P

Figure 6. miR-27 and miR-195 targeted the 3’-UTRs of PPARγ and FASN. (A) Bioinformatic analysis showed that the 3’-UTRs of PPARγ and FASN had binding sites in the sequences of miR-27 and miR-195. (B, C) The DLRA was performed by co-transfection of luciferase reporter containing the WT or MU-binding sites of PPARγ and FASN with miR-27 and miR-195 mimics into cardiomyocytes. (DF) Isolated cardiomyocytes were transfected with NC, miR-27, and miR-195 inhibitors for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR and WB were utilized to detect the expression levels of PPARγ and FASN mRNA and protein in isolated cardiomyocytes in each group (n = 3). **P < 0.01 and ***P < 0.001 vs. the NC/normoxia group. ###P < 0.001 vs. the hypoxia group.