Figure 7. Effect of PPARγ and FASN overexpression on hypoxia-influenced MR. Isolated cardiomyocytes were transfected with pcDNA3-NC, pcDNA3-PPARγ, and pcDNA3-FASN for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR was performed to detect the mRNA expression levels of (A) PPARγ, (B) FASN, (C) SIRT1, (D) FOXO1, (E) SREBP1c, (F) ACLY, (G) ACACB, (H) ADPN, (I) ATGL, and (J) GLUT4 in the isolated cardiomyocytes in each group (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the normoxia group. #P < 0.05 and ##P < 0.01 vs. the hypoxia group.