Research Paper Volume 13, Issue 7 pp 10158—10174

Hypoxia-induced miR-27 and miR-195 regulate ATP consumption, viability, and metabolism of rat cardiomyocytes by targeting PPARγ and FASN expression

Effect of PPARγ and FASN overexpression on hypoxia-influenced MR. Isolated cardiomyocytes were transfected with pcDNA3-NC, pcDNA3-PPARγ, and pcDNA3-FASN for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR was performed to detect the mRNA expression levels of (A) PPARγ, (B) FASN, (C) SIRT1, (D) FOXO1, (E) SREBP1c, (F) ACLY, (G) ACACB, (H) ADPN, (I) ATGL, and (J) GLUT4 in the isolated cardiomyocytes in each group (n = 3). *P #P ##P

Figure 7. Effect of PPARγ and FASN overexpression on hypoxia-influenced MR. Isolated cardiomyocytes were transfected with pcDNA3-NC, pcDNA3-PPARγ, and pcDNA3-FASN for 24 h followed by hypoxia treatment for 24 h. Cells under normoxia served as controls. RT-qPCR was performed to detect the mRNA expression levels of (A) PPARγ, (B) FASN, (C) SIRT1, (D) FOXO1, (E) SREBP1c, (F) ACLY, (G) ACACB, (H) ADPN, (I) ATGL, and (J) GLUT4 in the isolated cardiomyocytes in each group (n = 3). *P < 0.05, **P < 0.01, and ***P < 0.001 vs. the normoxia group. #P < 0.05 and ##P < 0.01 vs. the hypoxia group.